rabbit polyclonal anti-human calnexin  (Santa Cruz Biotechnology)

Journal: The Journal of Biological Chemistry

Article Title: Characterization of Caenorhabditis elegans sphingomyelin synthases through heterologous expression

doi: 10.1016/j.jbc.2025.110300

Figure Lengend Snippet: Sub-cellular localization of C. elegans SMS family members in HeLa cells. Confocal immunofluorescence microscopy of HeLa cells transfected with V5-tagged C. elegans SMS family members and co-stained with antibodies against the V5 epitope and ER marker calnexin or Golgi marker GM130. Representative images are shown for HeLa cells transfected with ceSMSα-V5 ( A and B ), ceSMSβ-V5 ( C and D ), ceSMSγ-V5 ( E and F ), and ceSMSr-V5 ( G, H ). Bar, 10 μM. See Methods Section 5.9 for imaging details.

Article Snippet: The following antibodies were used in this study: rabbit polyclonal anti-human calnexin (Santa Cruz), rabbit anti-drosophila calnexin (Abcam Ltd), mouse monoclonal anti-Golgi130 (BD Biomedicals), mouse/rabbit polyclonal anti-V5 antibodies (Sigma), rabbit polyclonal anti-biotin (Rockland), and mouse monoclonal anti-d120kd (EMD).

Techniques: Immunofluorescence, Microscopy, Transfection, Staining, Marker, Imaging

Journal: Biochemical and biophysical research communications

Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

doi: 10.1016/j.bbrc.2024.151269

Figure Lengend Snippet: Fig. 3. CHKA mediates exosomes secretion in glioma cells. A-C Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in shCHKA and shNC group along with the negative control protein Calnexin. D Exosome protein concentration in the shCHKA and shNC groups of glioma cells using the BCA assay. E Electron microscopy was performed on shCHKA and shNC cells in glioma cells (Scale bars = 1 μm). F The number of multivesicular bodies (MVBs) per glioma cell profile. G The number of intraluminal vesicles (ILVs) per MVB profile in glioma cells. *P < 0.05, **P < 0.01, ***P < 0.001. shCHKA: CHKA knockdown group, shNC: con trol group.

Article Snippet: Antibodies used in the Western blotting process included: rabbit antihuman CHKA polyclonal antibody (abcam, USA), rabbit anti-human GAPDH polyclonal antibody (Affinity, China), rabbit anti-human CD63 polyclonal antibody (Proteintech, China), rabbit anti-human TSG101 polyclonal antibody(Proteintech, China), rabbit anti-human calnexin polyclonal antibody(Proteintech, China), rabbit anti-human LC3B polyclonal antibody(Sigma, USA), and rabbit anti-human P62/SQSTM1 polyclonal antibody(Sigma, USA).

Techniques: Western Blot, Marker, Isolation, Negative Control, Protein Concentration, BIA-KA, Electron Microscopy, Knockdown

Journal: International Journal of Molecular Sciences

Article Title: Comprehensive Analysis of Exosomal MicroRNAs Derived from UVB-Irradiated Keratinocytes as Potential Melanogenesis Regulators

doi: 10.3390/ijms25063095

Figure Lengend Snippet: Exosomes derived from UVB-irradiated keratinocytes are isolated and characterized by their size. ( A ) Confirmation of exosome specific markers such as CD9 and CD81 using Western blot. Negative exosomal marker calnexin was lost in the samples. ( B ) Exosome size distribution measured using electrophoretic trace. ( C ) Size distribution of microvesicles (nm) assessed using nanoparticle tracking analysis. The NTA results of keratinocyte cell-derived exosomes showed a 114 ± 6.5 nm mode size of the exosomes.

Article Snippet: The antibodies used were as follows: B-actin antibody (Sigma, St. Louis, MO, USA), MITF antibody (ThermoFisher Scientific, Fremont, CA, USA), rabbit anti-tyrosinase (Abcam, Cambridge, UK: ab170905), rabbit anti-TRP1 (Abcam, ab178676), rabbit anti-TRP2/DCT (Abcam, ab221144), anti-CD63 antibody (mouse monoclonal to CD63, ab59479), anti-human CD9 IgG rabbit polyclonal (System Biosciences, Palo Alto, CA, USA), and anti-human calnexin rabbit polyclonal (Cell signaling Technology, Danvers, MA, USA, Beverly; National Institute of Health, Bethesda, MD, USA).

Techniques: Derivative Assay, Irradiation, Isolation, Western Blot, Marker